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Collaboration › Details

Shimadzu–Perfinity: protein sample preparation, –201103 collab co-development Perfinity Workstation for LC-MS sample prep

 

	Period	2011-03-11
	Partner, 1st	Shimadzu Scientific Instruments Inc. (SSI)
	Group	Shimadzu (Group)
	Partner, 2nd	Perfinity Biosciences Inc.
	Product	Perfinity Workstation (Automated Protein Sample Prep)
	Product 2	LC/MS system

Shimadzu Scientific Instruments, Inc.. (3/11/11). "Press Release: New Perfinity Workstation Automates Protein Sample Preparation".

Enables users to start with serum and have peptides ready for LC/MS analysis in as little as 10 minutes

Scientists know that the most important part of mass-spec analysis is the preceding separation process. That's why Shimadzu Scientific Instruments, in partnership with Perfinity Biosciences Inc., has developed the Perfinity Workstation, a multi-column apparatus that automates protein separations and mass spec sample preparation for more efficient LC/MS analysis.

The Perfinity Workstation uses utilizes Shimadzu hardware components in conjunction with five Perfinity Optimized columns that each perform a step of the mass-spec sample-preparation process - affinity selection, buffer exchange, digestion, desalting and reverse-phase separation. The automated integration of these steps enables users to start with serum and have peptides ready for LC/MS analysis in 10 minutes. The automated process removes much of the equipment and labor associated with mass-spec analyses of proteins.

The Perfinity Workstation combines the selectivity of antibodies with the resolving power of chromatography. Buffer exchange and desalting are performed on-line. The reduction in sample-processing time enables users to screen a variety of conditions rapidly.

The major advantage of the Perfinity Workstation's sample-preparation approach is that users are not required to immobilize antibodies. Instead, scientists can add antibodies directly to the sample. By removing the immobilization process, users have eliminated a potential source of error, making LC/MS analysis with Perfinity Workstation more accurate and reliable.

This platform is customizable across several application areas, including assay development, protein purification, drug development and biomarker discovery. For example, methods built into the workstation automate core steps of assay development, limiting the amount of protocol optimization required.

Record changed: 2012-04-07

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• [2] Shimadzu Corporation. (5/11/12). "Press Release: Presentation – Operating Results and Financial Position Ended March 2012. President & CEO Akira Nakamoto". Kyoto....
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• [4] Shimadzu Corporation. (5/10/12). "Press Release: Operating Results and Financial Position For the Fiscal Year Ended March 31, 2012". Kyoto....
• [5] [iito] Business Intelligence. (4/20/12). "[MSC] Newsletter March 2012". Bremen....
• [6] Shimadzu Scientific Instruments, Inc.. (4/9/12). "Press Release: Shimadzu Scientific Instruments and the University at Texas at Arlington Form Shimadzu Center for Advanced Analytical Chemistry"....
• [7] University of Texas at Arlington. (4/6/12). "Press Release: Shimadzu Gift Brings Millions in Scientific Equipment to UT Arlington"....
• [8] Shimadzu Corporation. (4/4/12). "Press Release: »Excellence in Science« Established as Brand Statement for Shimadzu Group"....
• [9] Sony DADC. (3/21/12). "Press Release: Shimadzu/Kratos and Sony DADC Sign New Agreement to Extend Collaboration". Manchester....
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