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Shimadzu Corporation. (12/5/12). "Press Release: New Platform for Automated Protein Analysis – Perfinity Integrated Digestion Platform (iDP) Enables Automatic Enzymatic Digestion of Proteins in One Minute, Improving Protein Pretreatment Efficiency".
|Organisation 2||Perfinity Biosciences Inc.|
|Product||Perfinity iDP system (Integrated Digestion Platform)|
|Product 2||LC/MS (liquid chromatography/mass spectrometry)|
|Index term 2||Shimadzu–Perfinity: protein sample preparation, 201212– global marketing + distribution of Perfinity iDP system by Shimadzu|
Shimadzu has released the Perfinity iDP (Integrated Digestion Platform) system. An online enzyme digestion HPLC system, Perfinity iDP fully automates the protein analysis workflow, from protein digestion to HPLC separation and MS detection.
In partnership with Perfinity Biosciences, Inc., Shimadzu Scientific Instruments, American Subsidiary of Shimadzu Corporation released this product in North America in this March. This announces the launch of the Perfinity iDP in Japan and other countries.
Perfinity iDP features a proprietary, optimized immobilized trypsin column that significantly reduces the digestion time compared to conventional manual methods. The processes from enzymatic digestion to desalting and HPLC analysis of digested peptides are performed online, which enables fully automatic processing, reduces pretreatment work, and ensures reliable pretreatment and analysis. This new platform offers (1) online trypsin digestion of proteins in as little as one minute, (2) dedicated software to support method creation, and (3) online automation for reliable, reproducible results. These features allow it to support a wide range of applications from peptide mapping in protein preparations and antibody drugs to biomarker validation.
* Perfinity iDP is registered trademark of Perfinity Bioscience, Inc. in the United States.
Background to the Product Development
The increased demand for protein preparations, antibody drugs, and protein biomarkers has created the need for an efficient LC/MS or HPLC analytical method for digested peptides obtained from the enzymatic digestion of proteins. Generally, proteins are digested in enzymes over several hours or a day, passed through solid-phase extraction and re-dissolving processes, and then introduced into an LC/MS or HPLC for analysis. This method takes an extremely long time for enzymatic digestion and requires pretreatment processes to be handled manually, which places a great workload on the analyst and may lead to poor results due to human errors. In contrast, the Perfinity iDP system offers extremely rapid enzymatic digestion and fully automates the series of processes up to HPLC separation to improve the efficiency of pretreatment and analysis, and ensure reliable analysis results.
Features of the Product
(1) Automatic trypsin digestion of proteins in as little as one minute
The proprietary high-efficiency immobilized trypsin column and optimized trypsin digestion buffer solution achieve highly efficient digestion of proteins in as little as one minute. Moreover, parallel processing of the stages from trypsin digestion to concentration in the desalting column and the stages from elution out of the desalting column to HPLC analysis improve analytical throughput, allowing analysis of up to 200 samples per day.
(2) Dedicated software supports method creation from pretreatment to analysis
Dedicated software supports method creation and selection, eliminating the need to make complex parameter settings. When analysis is complete, the Perfinity iDP software can access the LabSolutions software to easily browse and analyze the results.
(3) Online automation achieves reliable, reproducible results
In contrast to conventional manual operations, the Perfinity iDP offers more uniform control across the board from enzymatic reactions to concentration and desalting, which reduces human errors and improves the reproducibility of the results. The extraordinarily low levels of carryover ensure a highly reliable analysis environment, even when an LC/MS detector is used.
Record changed: 2016-03-19
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