ultraflex™ TOF/TOF mass spectrometer

Web link http://www.bdal.com/products/maldi/ultraflextreme-series/ov [...]

	Next higher product group	MALDI-TOF mass spectrometer
	Status	2001-07-29 sales start
	Price	USD 575,000 (price 2001-07-29)
	Document for price	Bruker Daltonics, Inc.. (7/29/01). "Press Release: Bruker Daltonics Introduces Ultra-sensitive MALDI-TOF/TOF System at the 15th Protein Society SymposiumConference". Philadelphia, Pens..
	Organisation	Bruker Daltonics Inc.
	Group	Bruker Corporation (Group)

Bruker Daltonics, Inc.. (7/29/01). "Press Release: Bruker Daltonics Introduces Ultra-sensitive MALDI-TOF/TOF System at the 15th Protein Society SymposiumConference". Philadelphia, Pens..

Bruker Daltonics Inc. (NASDAQ: BDAL) today announces its new UltraFlex™TOF/TOF system for ultra-sensitive high-throughput proteomics at the 15th Symposium of the Protein Society. The UltraFlex TOF/TOF is expected to ship in Q4 of this year, and will be priced at $575,000.

The UltraFlexTOF/TOF is a patented MALDI tandem time-of-flight (TOF/TOF) mass spectrometer specifically designed for the highest sensitivity and minimal sample consumption. The UltraFlexTOF/TOF enables very high-throughput protein identification by MALDI-TOF peptide mass fingerprinting, immediately followed on the same AnchorChip™ MALDI-target by more detailed MS/MS protein characterization.

This should dramatically increase the throughput of protein identification and characterization. The innovative UltraFlexTOF/TOF requires typically only 50-100 laser shots per MS/MS spectrum, and therefore consumes very little sample. For example, the UltraFlexTOF/TOF can measure an MS/MS spectrum from 500 attomol of a peptide with only 50 shots, resulting in comprehensive fragment ion coverage from low mass immonium ions all the way to the precursor ion. Alternative MALDI-TOF/TOF designs appear to take several thousand shots for each MS/MS spectrum, thus requiring tens of thousands of shots for a typical 10-20 MS/MS spectra per sample.

We estimate that for realistic tryptic peptide samples from gels, the UltraFlexTOF/TOF can acquire 10-20 MS/MS spectra from 5-10 femtomol of sample present on the MALDI target (not sample consumed!) in just a few minutes total.

The UltraFlexTOF/TOF benefits from Bruker Daltonics' leadership in high-throughput MALDI-TOF technology aimed at industrial biology. Our MALDI targets have industry-standard microtitre plate (MTP) format, and are thus easily compatible with standard sample preparation robots. An optional robot is available for automated delivery of additional MTP MALDI targets into the UltraFlexTOF/TOF for unattended 24/7 operation.

Our high-throughput ruggedized xy-table, which has been developed for our autoflex® MALDI-TOF product for measurements of up to 30,000 SNP samples per day, has also been implemented in the UltraFlexTOF/TOF. This unique xy-table can move from target spot to target spot in 0.4 seconds in reliable 24/7 operation with unsurpassed positional accuracy. The new UltraFlexTOF/TOF electronics enables automated system self-testing, as well as remote diagnostics and service.

The UltraFlexTOF/TOF fully incorporates our proprietary AnchorChip technology, which improves MALDI-TOF sensitivity typically by 1-2 orders of magnitude. AnchorChips also have the additional throughput advantage that they "anchor" the exact sample location and size on the target for robust and fast automation without time-consuming searches for samples or "sweet spots".

Importantly, the UltraFlexTOF/TOF also offers uncompromised standard MALDI-TOF MS performance. In fact, it has the same sub-femtomol sensitivity and same resolution (>25,000) as our high-performance reflexIV MALDI-TOF system, which owes its superior sensitivity to a completely gridless ion source and patented gridless reflector design.

"We believe that our UltraFlexTOF/TOF has unsurpassed sensitivity and throughput, both of which are absolutely essential in industrial-scale proteomics," commented Dr. Frank H. Laukien, President and CEO. "We expect that with only about 100 laser shots per precursor peptide, we can do MALDI-TOF/TOF analysis on dozens of tryptic peptides per gel spot without running out of precious protein sample. Simply put, we believe we have the best available technology for a dramatic acceleration of protein identification and characterization."

COMPLETION OF PROTEINEER™ SUITE FOR PROTEOMICS

At the Protein Society Symposium, Bruker Daltonics also introduces Proteineer DP Digest & Prep station, a new gel spot digestion robotics workstation for proteomics. The Proteineer DP Digest & Prep station completes our Proteineer suite, an integrated comprehensive solution for protein identification and characterization after 1d- or 2d- gel electrophoresis separation. It complements the previously introduced Proteineer SP Spot Picker robotics workstation for imaging of gels, visual analysis, spot selection, and cutting spots out of 2D gels used for protein separations.

-- The Proteineer DP automates and simplifies some of the remaining tasks in sample preparation prior to MALDI-TOF or ESI-ion trap analysis of proteins:

-- Proteineer DP is based on our successful map™ II/8 MALDI AutoPrep system, and adds an automated station for tryptic digestion of protein spots cut from gels.

-- The resulting tryptic peptide samples are automatically cleaned up for optimal results prior to MALDI-TOF or ESI-ion trap analysis.
-- After clean-up, the digest samples are automatically deposited on our AnchorChip targets for ultra-sensitive MALDI-TOF analysis, or

-- the samples are prepared in 96 or 384-well plates for subsequent analysis by our esquire3000plus™ ion traps.

Our Proteineer suite of consumables, robotics, mass spectrometry systems and bioinformatics modules for proteomics offers the unique combination of the two most powerful mass spectrometry methods for protein identification and characterization, MALDI-TOF and ESI-ion trap mass spectrometry. Specifically, Proteineer offers an integrated solution for adaptive intelligent acquisitions strategies in protein identification and characterization with

-- our leading ultra-high throughput MALDI-TOF systems as a first very high-throughput protein identification step,

-- optionally, our new UltraFlexTOF/TOF for high-throughput MS/MS as a second step for protein characterization,

-- robust high-performance ion trap ESI/MSn as a third step for the most detailed protein characterization, such as the determination and localization of post-translational modifications, increased sequence coverage, the identification of proteins in complex mixtures, and protein quantitation, as well as

-- ProteinScape™, our bioinformatics software package for comprehensive proteomics data warehousing, and analysis of all the data generated in the entire Proteineer workflow.

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ABOUT BRUKER DALTONICS

Bruker Daltonics is a leading developer and provider of innovative life science tools based on mass spectrometry. For more information about Bruker Daltonics, please visit http://www.bdal.com/.

CAUTIONARY STATEMENT

All statements in this press release that are not historical are forward-looking statements within the meaning of the Securities Exchange Act of 1934, as amended, including, but not limited to, statements regarding Bruker Daltonics' "expectations," "beliefs," "hopes," "intentions," "strategies" or the like. Such forward-looking statements are subject to risks and uncertainties that could cause actual results to differ materially for Bruker Daltonics from those projected, including, but not limited to, risks and uncertainties relating to technological approaches, product development, manufacturing, market acceptance, cost and pricing of Bruker Daltonics' products, dependence on collaborative partners, suppliers, FDA and other regulatory approvals to the extent applicable, competition, the intellectual property of others, patent protection and litigation and other risk factors discussed from time to time in Bruker Daltonics' reports or filings with the Securities and Exchange Commission. Bruker Daltonics expressly disclaims any obligation or undertaking to release publicly any updates or revisions to any forward-looking statements contained herein to reflect any change in Bruker Daltonics' expectations with regard thereto or any change in events, conditions, or circumstances on which any such statements are based.

FOR FURTHER INFORMATION:

Please contact: Dr. Tim Alavosus, Marketing Manager Tel: (978) 663-3660, ext. 1339 Email: tim.alavosus@bdal.com

For investors: Michael Willett, Investor Relations Officer Tel: (978) 663-3660, ext. 1411 Email: michael.willett@bdal.com

Record changed: 2011-11-25

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ultraflex™ TOF/TOF mass spectrometer

Bruker Daltonics, Inc.. (7/29/01). "Press Release: Bruker Daltonics Introduces Ultra-sensitive MALDI-TOF/TOF System at the 15th Protein Society SymposiumConference". Philadelphia, Pens..

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