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Shimadzu Corporation. (12/10/09). "Press Release: A Bridge with Our Customers. Sawai Pharmaceutical Co., Ltd. and Takasago International Corporation".

Region Region Japan
Organisations Organisation Shimadzu Corporation
  Group Shimadzu (Group)
  Organisation 2 Sawai Pharmaceutical Co., Ltd.
Products Product LCMS-IT-TOF hybrid mass spectrometer (Shimadzu)
  Product 2 GC/MS system

What customers want from Shimadzu products
- A bridge with our customers -

This corner features visits to customers by Shimadzu's R&D engineers. Here we document the comments and requests of the customers in response to questions about newly developed products and our unique technologies. Working to provide products and services that are suitable for the needs of our customers as business partners, Shimadzu Corporation is endeavoring to obtain feedback from customers in Japan and abroad with the aim of promoting new product development and technology improvements.
High-speed Liquid Chromatograph Mass Spectrometer

Sawai Pharmaceutical Co.,Ltd.
Spoke with Mr. Yagi of Sawai Pharmaceutical Co., Ltd. regarding Shimadzu's LCMS-IT-TOF system.
Advanced Flow Technology Series Multi-Dimensional GC/GCMS System

Takasago International Corporation
Spoke with Mr. Yaguchi of Takasago International Corp. regarding Shimadzu's multidimensional GC/GCMS system.

A bridge with our customers
High-speed Liquid Chromatograph Mass Spectrometer

Assistant Senior Researcher
Analytical Research Department
Analysis III Group
Sawai Pharmaceutical Co.,Ltd.

Mr. Yagi's group has several high-end LC/MS systems, in addition to NMR and IR analytical instruments, for conducting structural analysis of organic compounds. The Shimadzu LCMS-IT-TOF is one of the tools they are using for identification of impurities in the compounds.

How is Shimadzu's LCMS-IT-TOF system being used?

These days, generic drugs are considered to be of "high quality and high purity." Purity is demanded for both drug ingredients and products. Since impurities of unknown structures will most likely be detected in LC analysis, and the source of these impurities cannot be determined at that stage, the objective shifts to structural prediction. Suppressing the generation of impurities becomes possible if the structure of impurities can be predicted, enabling the manufacture of high-quality drugs. Although we have used ion trap type MS instruments previously, our company selected the LCMS-IT-TOF for structural prediction of unknown compounds present at micro concentrations.

Why did you select the LCMS-IT-TOF as your final choice?

Value and performance. Since an FTMS would be an order of magnitude more expensive, even though it might be worth it, we honestly couldn't spend that much. Once a budget was determined, it came down to performance, and our wish to obtain accurate mass and conduct MS/MS measurement in one measurement operation. We made a decision between your company's IT-TOF and another company's Q-TOF. Based on the best balance of performance and value, we selected the LCMS-IT-TOF.

So you made your selection based on careful consideration of cost and performance. Thank you. Regarding the actual conditions under which you are using the instrument, about how many people are using the LCMS-IT-TOF at your company?

We find that a group of 6 people can handle the required operations without the need for a group leader.

Instruments for conducting structural analysis have become fairly easy to use these days. The difference between this instrument and the ion trap that is conventionally used is accurate mass measurement, but aside from that, are there any other advantages or benefits you have found with the LCMS-IT-TOF?

Although this might not apply to every recent instrument, polarity switching is not possible with conventional instruments, and scan speed is slower compared to the IT-TOF. Since there is no way to know under which conditions measurement of an unknown compound should be conducted, that unknown compound may be detected in positive mode or in negative mode, making it very likely that the measurement will have to be repeated. Furthermore, though not a problem if it is the major component, when it comes to separating a component that makes up just 0.1% or 0.05% of the sample, a longer column becomes necessary, which increases the analysis time. With the LCMS-IT-TOF, measurement which previously required two runs can now be completed in one run. In addition, the ability to conduct accurate mass measurement is, after all, a big point. The most important factor is the ability to perform MS/MS accurate measurement with the IT-TOF. Since accurate mass can be determined for neutral loss, etc. when conducting structural analysis, it is now possible to see what is separated at that time.

Even with a neutral loss of only 28, if it is only determined as an integer, about three composition candidates can easily be considered, including CO, C2H4 and N2. So, the advantages can be listed as polarity switching, the ability to obtain MS/MS accurate mass spectra, and scan speed.

Next, what kind of LC analysis conditions do you normally use?

For the mobile phase, we use the typical solutions, such as aqueous formic acid, acetonitrile, TFA, etc. Perhaps we use formate mobile phases most of all. After we first verify separation using the LC method, we shift to the MS method. For a typical drug, a phosphate method is most often used. We also use ion pair methods, but since they cannot be used as is, we must change the conditions to search for those that will achieve the same level of separation. Although this is quite a time-consuming process, it is the first stage of our MS analysis. Unless we go through this process, even if we determine the structure of the eluted substance using MS analysis, once it is handed off to the analysis section, we will not know where the substance elutes using a different method. If this were actually to occur, determination of the structure would have no meaning. Since it is essential that we know that the substance which elutes at a specific position using a specific method has a particular structure, it is essential to first complete the task of developing a method that generates the same kind of results.

And there is a possibility that elution order may change if the method is changed.

That does occur. In such a situation, we look at UV spectra taken with a PDA detector, and verify the separation while looking for shifts of the target contaminant after changing the method. So, it takes quite a bit of time to establish the method before the process can move to the MS stage. Anyway, rather than having a shorter analysis time, it is preferable that the substances elute in about the same order. That's because even if the retention times are slightly off, they can be moved.

For your department, specialized software or some solution capable of transferring a method to an LC/MS would be very useful. And it would be nice if the LC method could be used as it is in MS analysis.

Yes, that would be the best solution. There are supposedly MS manufacturers who claim that phosphate can be used, but I believe that is absolutely not possible.

May I suggest something? First, have you ever heard of the Co-Sense system? It is a system in which, for example, the phosphate mobile phase being used in the LC analysis is substituted for another mobile phase while trapping the peak. Do you think you might have some interest in such a system?

I have briefly seen such a Co-Sense system for NMR. Is the system you are referring to applicable to MS?

It is a system that prevents the phosphate buffer solution from reaching the MS. I would be pleased to show it to you if there is an opportunity.

The LCMS-IT-TOF system that you are using includes several types of software, such as composition prediction software and a program that compares chromatograms. Could you give me your opinion on the advantages of the data analysis software overall and any suggestions you might have regarding these products?

To tell the truth, I have not used all of these yet. I have used the composition prediction program of LCMSsolution.

Are the search result composition formulas of impurities listed in order of the highest probability?

Yes. I believe this is more or less the case.

Could you explain what kinds of advantages the LCMS-IT-TOF has in comparison to structural analysis using NMR and IR?

The biggest advantage is the ability to measure samples that do not consist of a single constituent. The fact that you can see components in their mixed state in a sample, even at low quantities, is a big plus. Greater quantities are required with NMR and IR, and unless it is a solitary constituent substance, you don't know what you are looking at. Simply put, a sample consisting of a single solitary substance is not necessary. Plus, sensitivity is good, making it especially applicable for looking at micro levels of impurities.

So the workflow is to first convert the LC method into one that can be used for LC/MS, then measure everything by IT-TOF and obtain the predicted structure. And if the structure cannot be predicted by MS within that process, the process would be extended by collecting fractions and conducting supplemental IR and NMR measurement.

Yes, that's correct. The workflow starts with fraction collection, or if structural prediction can be accomplished, conversely we will try to conduct synthesis. The product synthesized by that process is then measured by NMR and IR, and the sample fraction is also measured by NMR and IR to complete the determination of the structure as the final step

For structural analysis, you have MS, NMR and IR instruments. Among these, would you say that the LCMS-IT-TOF is the most frequently used?

Yes, that is so. Since it is at the most upstream position in the workflow, the analysis all starts with LCMS-IT-TOF measurement.

Since you've started using the LCMS-IT-TOF for accurate mass measurement, has structural prediction become quicker?

Yes, I believe it has become quicker. Previously, we had to first think about the composition formula to determine what it is, even if it had a large molecular weight. Now, it is possible to quickly narrow the possible composition formula candidates, allowing the process to quickly proceed to determining where and what kind of change has occurred, thereby speeding things up.

Are there any samples or measurement methods you would like to shift your attention to?

Up to now we have only been dealing with low molecular weight compounds, but I believe this could change. If we shift from low molecular weight compounds to antibody drugs and nucleic acid drugs, we will need to obtain the skills to address these substances. We can obtain good instruments if we spend as much money, but I think it is necessary for us to acquire the skills to handle those new substances.

If you have any final comments about the system as a whole, I would certainly like to hear them.

I think it is good that we acquired this system. At first, we talked about conducting measurement up to a certain point with the existing instruments, and then using the IT-TOF to obtain a little more detailed information to finish off the measurement. But actually, everyone seems to use the LCMS-IT-TOF from the start of the analysis if it is available: if unavailable, they use the old instrument because it cannot be helped. With the ability to obtain plenty of data and measure accurate mass, the work can be completed without having to repeat operations two or three times. It is considerably useful.

You have been using the LCMS-IT-TOF for about 6 months. How often have you performed maintenance?

We haven't had to do any maintenance at all. The maintenance we performed consisted of just wiping the source once or twice. During that time there were absolutely no problems with sensitivity, which remains stable without dropping at all. Also, the spray nozzle has never become clogged, and there has never been a problem of ions not emerging.

Finally, then, please share your opinions about our company's support, about us as an analytical instrument manufacturer overall, and if there is any other way in which we can help you.

It would be very helpful if you could provide service promptly. For example, if we call you in the morning, it would be ideal if you could arrive before noon, but I suppose it would be difficult for any company. I assume that there are still not enough people that can support the IT-TOF.

I also would like to increase the number of engineers for the LCMS-IT-TOF. The number of LCMS-IT-TOF instruments delivered to Europe, America, Asia and South America now outnumber those in Japan. Shimadzu is a Japanese manufacturer, and our engineers must at times travel abroad. As overseas engineers become more knowledgeable with respect to LCMS-IT-TOF, I believe the workload of Japanese engineers will decrease and service will be more promptly provided.

Comment from Head of Development Team

The 0.1-second high speed polarity switching which was mentioned as one of the salient features of the LCMS-IT-TOF was the most difficult hurdle the engineers faced in the development process. We once thought it was impossible to achieve polarity switching in just 0.1 second while maintaining high accuracy in both positive and negative modes. The members of the development team are extremely pleased and proud that this very feature has become the one that customers value.

A bridge with our customers
Advanced Flow Technology Series Multi-Dimensional GC/GCMS System

Senior Scientist
Analytical Technology Resarch
Corporate Research &
Development Division
Takasago International Corporation

When Shimadzu was developing the multidimensional (MD) GC, the staff at Takasago International Corp. provided great assistance in helping us complete development of the system by evaluating the instrument, not only from a functional standpoint, but in other aspects as well, including convenience and instrument stability. We talked to Mr. Yaguchi, who is fully utilizing the MDGC in the research of optically active fragrances, etc., regarding the hardships he encountered while the instrument was being developed, and the status of the instrument in its present utilization.

What prompted your company to request the development and customization of the multidimensional GC system (MDGC) in 2003?

The original MDGC we were using was commonly used in Europe at that time for fragrance analysis. However, it was a very complicated instrument, and unless someone was very familiar with its operation, it was very difficult to use effectively. Changing the column and other basic instrument maintenance required a lot of time and technical expertise. After years of use, it finally deteriorated, so it was necessary to replace it with another MDGC. By that time I wanted an instrument that would be easier to use from the standpoints of both hardware and software, so I looked for a manufacturer who would be willing to accept special customization requests.

So you approached Shimadzu with your proposal, and we decided to create an instrument together. We modified various types of hardware and switching elements many times, and your company actually used and evaluated them. We also custom designed the software to make it easy to use with MDGC. Can you tell us about any of the hardships you encountered at that time?

Once, I set up a 10-line batch analysis, started the analysis and went home for the night. When I arrived at work the next day, I found that the analysis had stopped after completing only 3 lines of the batch analysis. That was a severe blow, because we were really pressed for time. On-going consultation with the technicians as to what might be causing interruption of the batch analysis gradually resulted in improved operation. Of course, problems like the unexpected interruption of analysis no longer occur.

There was a considerable difference in the hardware and software from the time of initial development to when the MDGC was completed. In what way were these changes favorable?

Since the MDGC software is based on the improved Solution series of Shimadzu's workstation, it is extremely easy to use. There was absolutely no sense of discomfort with the new MDGC software because of our familiarity with the Solution series software gained through our long experience with the single-column GC and GC/MS software.

Is there anything further that you think should be improved in the present MDGC system?

I think there is sufficient functionality already built into the system. There may be some minor improvements that could ease routine operations, for example, more durable screw threads, etc. But as a system, it has no shortcomings and is easy to use.

Then, you could say that its degree of completion is high?

Yes, that's correct.

Thank you. In what kind of configuration are you using it presently? And, if possible, could you tell me about the purpose of your analysis?

In our configuration, the first step consists of an FID detector with switching between an AOC-20i (autoinjector) and a TD (thermal desorption system). The second step is used with an MS attached. The system is often used for qualitative and chiral analysis. Since a chiral compound in a natural substance may not necessarily be a racemic compound, the MDGC is used to determine the ratio of the d form to the l form, for example, a ratio of 70:30 or 80:20, etc. There are many compounds that are known to have different aromas, depending on the content ratios of the d form and l form. For example, a variation of a compound consisting of 100% of the d form and another with 100% of the l form may have completely different aromas. Our company uses asymmetric synthesis and enzyme reactions to obtain optically active aroma blends referred to as Chiraroma®.

In other words, these optically active aromas are mixed to achieve aromas that are similar to natural ones. By the way, how do you use the single-column GC and GCMS as distinguished from the MDGC?

The single-column GCMS is used almost entirely for general-purpose and routine analysis with a fixed method, while the MDGC is mainly used for analyses in which the methods are constructed painstakingly, step by step. When we wish to obtain high-quality, more comprehensive data, we often rely on the MDGC.

When you say high-quality data, do you mean that the peaks are acquired with certainty? Or, do you mean peaks that are actually of high purity.

There is really not much to worry about when using a single-column GC and GCMS to analyze targets of high purity, but when analyzing fragrance compositions, as many as one or two hundred peaks may be detected. At that point, extracting only the targeted substance from those peaks and obtaining the necessary information requires painstaking fractionation and purification when using a single-column GC and GCMS. When looking at both stereoisomers (d form, l form) of target compounds in a natural substance, it is not practical to eliminate all the surrounding material before conducting the analysis. But it is extremely easy to obtain the targeted information from within a complicated mixture using the MDGC.

Could you tell me what kind of GC analytical conditions you are actually using?

We normally use a Wax series column with high polarity in the first step GC, while in the second step GC we use a column for separating chiral optical isomers when conducting chiral analysis. For qualitative analysis, we sometimes use this in combination with a relatively nonpolar column. Since there are compounds that have an extremely strong aroma even at low concentrations, it can be difficult to identify a compound in the course of qualitative analysis due to the intensity and quantity of the fragrance. In such a situation, identification may not be easy if analysis is conducted with the single-column GCMS, so we use it in conjunction with GC/O (sniffing) data.

Are you obtaining the kind of results you expected from the Shimadzu MDGC? Do you have any requests regarding any of our products you are using?

Although not directly related to the MDGC system, we can obtain very clean chromatograms, method creation is simple, and analysis of the acquired data is very well addressed with GCMSsolution, which is very easy to use. I would have to say that at this point, there are no deficiencies from the standpoint of functionality. Compared to the GCMS control software of other manufacturers, it is an easy-to-use system. In particular, the postrun analysis function is excellent. However, if there were something to say about GCsolution and GCMSsolution, I think there should be more uniformity between the two interfaces. There are quite a few differences in menu arrangement and rules in these two interfaces. A function that is present in one might not be present in the other, or they might be different, or perhaps, accessed from a different menu. Because of such inconsistencies, an analyst who frequently uses both software packages may be confused.

Thank you for your valuable comments. We will incorporate your advice in our future development. Regarding future product development, aside from existing products, is there any such product you would like to see available or any such item you wish could be developed?

My wish is for innovative GC technological development. I am looking forward to seeing the new, next generation GC technology, with new knowhow and new technology that is exciting enough to capture everyone's attention. Of course, I think Shimadzu's developers are capable of doing this.

Can you share with me your opinions and any requests of Shimadzu Corporation or regarding our analytical equipment overall?

There is so much that it is difficult. I think that the relationship between hardware and software is still very important. Even if the hardware is good, there is not much chance that users will be able to use it if only hardware specialists can figure out how to use it. So, it is very important that the interface be very approachable, that it is easy to understand, and that operation menus and commands be where one would expect them to be. Even if you search for what you want to do, not being able to find it is extremely stressful for a user. If the software is not user-friendly, regardless of the number of functions and high overall performance, there is no way to take advantage of these merits. Of course, it is good to carefully read the manual, but unless the operation menus and commands can be quickly and easily located, the software will be difficult to use. Yes, I believe that easy-to-use software is very important.

I hope, in the future, we can develop good products while relying on your frank opinions. I am proud that the MDGC became a fine product thanks to the assistance of your company. It is a pleasure working with you.

I hope to be of help to you. Thank you very much.

Roses were in full bloom at the rose garden on Takasago International's premises.

Comment from Head of Development Team

Development of this instrument originated with an inquiry from Takasago International in 2003, asking if Shimadzu would be interested in developing an instrument capable of better heart-cut analysis. Since fragrance companies have some of the most demanding applications, it was necessary to pay very close attention to design of the hardware; design of the complex flow line was very tough. In addition, it was essential that easy-to-use software be developed. In particular, the requirement that MDGCsolution control two Solution software modules simultaneously made development extremely difficult, with the items to be checked increasing exponentially. In the end, the completed system featured both hardware and software that are superior to those of other companies, and the hardship encountered during development only served to deepen our sense of reward. In the future we will make further efforts to develop products that are easy to use for customers.

Record changed: 2019-06-09


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